By John H. Luft (auth.), James K. Koehler Ph. D. (eds.)
The prior decade has noticeable a amazing bring up within the use of electron microscopy as a researm instrument in biology and drugs. hence, so much institu tions of upper studying now boast a number of electron optical laboratories having numerous degrees of class. education within the regimen use of elec tron optical apparatus and interpretation of effects isn't any longer limited to some prestigious facilities. nonetheless, temniques used by researm employees within the ultrastructural area became tremendous different and intricate. even supposing a number of rather very good volumes of electron microscopic temnique at the moment are devoted to the fundamental parts to be had whim enable the beginner to obtain an inexpensive creation to the sphere, really few books were dedicated to a dialogue of extra advert vanced temnical points of the paintings. It used to be with this view that the current quantity used to be conceived as a convenient reference for employees already having a few heritage within the box, as a data resource for these wishing to shift efforts into extra promising temniques, or to be used as a complicated direction or seminar advisor. material has been mosen rather at the foundation of pertinence to provide researm actions in organic electron microscopy and emphasis has been given these components whim appear destined to vastly extend in necessary ness within the close to future.
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Additional resources for Advanced Techniques in Biological Electron Microscopy
Cytochem. 13, 579-582 (1965). LEDUC, E. : Ultrastructural cytochemistry. Enzyme and acid hydrolysis of nucleic acids and proteins. J. biophys. biochem. Cytol. 10, 437-455 (1961). Embedding Media - Old and New 33 LEDUC, E. , BERNHARD, W. : Water-soluble embedding media for ultrastructural cytochemistry. Digestion with nucleases and proteinases. In The Interpretation of Ultrastructure. : R. HARRIS (Symp. Int. Soc. Cell BioI. Vol. 1),21-45. New York: Academic Press. 1962. LEDUC, E. : Recent modifications of the glycol methacrylate embedding procedure.
It was originally suggested that cellosolve (the mono methyl ether of ethylene glycol) would serve (PEASE, 1966 a). However, we have since felt that this solvent can have deleterious effects, and now when we wish to embed in an epoxy resin we pass the tissue through propylene oxide. For Vestopal we go first to propylene oxide and then through toluene. It should be noted that an inertly dehydrated specimen (or for that matter any other sort of a specimen) need never again be exposed to water during the staining and sectioning processes.
MERCER, E. : Applications of an epoxide embedding medium to electron microscopy. J. roy. micro Soc. 76, 159-161 (1956). : Polyesters and Their Applications. New York: Reinhold Publishing Corp. 1956. : Recent developments in methacrylate embedding.!. A study of the polymerization damage phenomenon by phase contrast microscopy. J. biophys. biochem. Cytol. , 3-14 (1956). BORYSKO. : A new technique for comparative phase-contrast and electron microscope studies of cells grown in tissue culture, with an evaluation of the technique by means of time-lapse cinemicrographs.