Download Advanced Techniques in Biological Electron Microscopy II: by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.) PDF

By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)

The use of the time period "advanced" within the name of this e-book is a bit ar­ bitrary and intensely a lot relative with appreciate to time. Many concepts that have been thought of on the "cutting aspect" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in various laboratories. you may cite freeze-fracture, cryothin sectioning, or certainly lots of the box of test­ ning electron microscopy as concrete examples. hence using the time period "ad­ vanced thoughts" has to be interpreted with reference to the current cutting-edge, and comes in handy merely in informing the capability reader that this quantity isn't really a primer for use as an preliminary advent into simple organic elec­ tron microscopy. many glorious volumes have crammed that area of interest long ago few years, and it's not meant that this modest publication be a whole com­ pendium of the sector. in addition, any constrained number of papers on advanc­ ed concepts inevitably displays the personal tastes and arbitrary whims of the editor, thereby apart from many both very important tactics which the a professional reader will comfortably establish. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated innovations which have been suggestion to symbolize complicated and but ba­ sically morphological equipment for gaining elevated ultrastructural informa­ tion from organic specimens. the current quantity, nonetheless, stresses suggestions which supply particular physicochemical information at the speci­ mens as well as the structural information.

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34, 633 - 639 (1969) Rigas, D. , Osgood, E. : Purification and properties of the phytohemagglutinin of Phaseolus vulgaris. J. BioI. Chern. 212,607 - 615 (1955) Rosenblith, J. , Ukena, T. , Yin, H. , Berlin, R. , Karnovsky, M. : A comparative evaluation of the distribution of concanavalin A-binding sites on the surfaces of normal, virally-transformed, and protease-treated fibroblasts. Proc. Natl. Acad. Sci. S. : Distribution of concanavalin A receptors on normal rat liver cells and Zajdela ascites hepatoma cells.

Therefore, it is the pur- 42 w. D. PERKINS and J. K. KOEHLER pose of this discussion to present methodologies for labeling antibodies with the markers hemocyanin and radioiodine for TEM examination. The labeling techniques to be considered are those most commonly used at the present time. Their advantages and disadvantages will be evaluated. In addition to antibody labeling procedures, the further use and processing of several kinds of labeled antibody will be discussed. The procedures discussed will include preparation of cell suspensions prior to reaction with labeled antibody, reaction of cells with labeled antibody, and specialized preparation of cells after reaction with labeled antibodies.

Physiol. 42 A, 269 - 276 (1972) Isselbacher, K. : Increased uptake of amino acids and 2-deoxY-D-glucose by virustransformed cells in culture. Proc. Natl. Acad. Sci. S. , Greaves, M. : Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens. Clin. Exp. Immunol. 9,483 - 498 (1971) Kabat, E. : A study of the purification and properties of ricin. J. BioI. Chem. 168,629 - 639 (1947) Kalb, A. : The separation of three L-fucose binding proteins of Lotus tetragonolobus. Biochim. Biophys.

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