By Donna H. Wang
Donna Wang and a panel of hands-on specialists describe in step by step element the entire significant molecular, biochemical, and sensible concepts now utilized in probing the renin-angiotensin method. those variety from using embryonic gene focusing on to create of mutant traces, to the practical research of elements of the renin-angiotensin procedure. complete and hugely sensible, Angiotensin Protocols interprets specialist wisdom into effortlessly reproducible equipment that allow all biomedical investigators effectively to discover the position of angiotensin in wellbeing and fitness and ailment this day.
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Extra info for Angiotensin Protocols (Methods in Molecular Medicine)
1. Studies of local renal tissue effect of a specific gene product that is normally expressed in the kidney by cell-type specific gene targeting vs chimeric mouse. Celltype specific gene targeting, if successfully performed, can selectively inactivate a gene of interest in the kidney, and not in other organs. However, some abnormal phenotypes in the kidney can change the environment surrounding the kidney via ECF volume change, and so on, and, hence, blood pressure and so on, which in turn secondarily induces other abnormal phenotypes within the kidney.
13, 181. 56. Pals, D. , Masucci, F. , and Denning, G. S. (1971) Specific competitive antagonists of the vascular action of angiotensin II. Circ. Res. 29, 664–672. 57. Brunner, H. , Laragh, J. , and Keenan, R. (1973) Angiotensin-II blockade in man by SAR1-Ala8-angiotensin II for understanding and treatment of high blood-pressure. Lancet 1, 1945–1948. 58. , Brunner, H. , Vaughn E. , and Laragh, J. H. (1973) Angiotensin-sodium interaction in blood pressure maintenance of renal hypertensive and normotensive rats.
Vasc. Surg. 12, 122–127. 16. Castleman, B. and Smithwick, R. H. (1943) The relation of vascular disease to the hypertensive state based on a study of renal biopsies from one hundred hypertensive patients. JAMA 121, 1256. 17. Brenner B. , Meyer T. , and Hostetter T. H. (1982) Dietary protein intake and the progressive nature of kidney disease: the role of hemodynamically mediated glomerular injury in the pathogenesis of progressive glomerular sclerosis in aging, renal ablation, and intrinsic renal disease.